Chem 738: Introduction to Mass Spectrometry

Spring 2024, 1 credit, Thursday 9:55 am, Room B355 Chemistry:   Instructor: Martha M. Vestling

Mass spectrometry has evolved into one of the leading technologies for biological and chemical research. It is widely used in genomics, proteomics, drug discovery, forensics, polymer characterization, and combinatorial chemistry.

Topics to be covered:

  • basic vocabulary of mass spectrometry
  • ionization options and instrument types
  • basic sample handling, common contaminants
  • databases
  • fragmentation and sequencing
  • separations before mass spectrometry: LCMS, GCMS
  • peptides and proteins, post-translational modifications
  • when to use stable isotopes
  • metabolite identifications
  • quantitative analysis

Lectures will mix the basics of mass spectrometry with examples of sophisticated applications. Grades will be based on attendance and a short paper.

Optional Texts:  

"Mass Spectrometry," John S. O. McCullagh and Neil J. Oldham, Oxford University Press, 2019.

"HPLC and UHPLC for Practicing Scientists," 2nd ed., Michael W. Dong, John Wiley & Sons, Hoboken, NJ, 2019.

"Mass Spectrometry for the Novice," John Greaves and John Roboz, CRC Press, 2013.

 "The Expanding Role of Mass Spectrometry in Biotechnology," 2nd ed., Gary R. Siuzdak, MCC Press, San Diego, 2006.